Parameters AMB DOX
Retention time (min) 5.86 3.60
Tailing factor 0.96 1.42
Resolution 3.23
Theoretical Plates 9412 8722
Detection limit (µg/ml) 0.02694 0.0732
Quantitation limit (µg/ml) 0.08165 0.2219
Accuracy(%) 99.10-100.96% 99.17-100.96
  Precision (%RSD)
Intra-day (n=3) 1.06-1.69 0.67-1.18
Inter-day (n=3) 1.47-1.84 0.59-1.12
Instrument precision  (%RSD) 1.0139 1.0604
Specificity Specific Specific
Table 3: Summary of validation parameters of developed HPTLC method.