Parameter Method condition % RSD of peak area
AMB DOX
Flow rate 0.8 ml/min 0.87 1.16
1.2 ml/min 0.94 1.28
Mobile phase ratio 0.02 M KH2PO4:Methanol (66:34) 1.46 1.29
(54:46) 1.09 1.56
Table 4: Results from the robustness study of Method.