Parameter
Method condition
% RSD of peak area
AMB
DOX
Flow rate
0.8 ml/min
0.87
1.16
1.2 ml/min
0.94
1.28
Mobile phase ratio 0.02 M KH
2
PO
4
:Methanol
(66:34)
1.46
1.29
(54:46)
1.09
1.56
Table 4:
Results from the robustness study of Method.