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Table 2: Final sample dilution factor as a result of two step purification. After methanol precipitation, additional sample dilution is caused by the necessity of reducing the content of organic solvent to the desired concentration (20% in this particular case, s=0.2). This “double dilution” results in very fast analyte content decline per sample volume, as given by the relationship: f=(1-S)(s/S). The values are reported without taking into account a correction for volumes non-additivity. |