| Analyte |
MRM Transitiona
(parent→quan, qual) |
LLOQ
(ng/ml) |
HLOQ
(ng/ml) |
Accuracy
range (%) |
Carryover
Limit
(ng/ml) |
Precision (level 1/2/3, n=30) |
| Mean (ng/ml) |
Total CV (%) |
Intra CV (%) |
| Buprenorphine |
468.3→396.3, 414.3 |
9.7 |
5647 |
80.5-113.0 |
10160 |
40/378/747 |
6.4/6.4/4.8 |
5.0/0.5/4.1 |
| Norbuprenorphine |
414.3→187.1, 340.1 |
9.6 |
4317 |
83.8-96.0 |
10443 |
32/326/613 |
3.3/3.3/10.3 |
2.2/0.2/9.9 |
| 6-MAM |
328.1→211.1, 193.1 |
4.9 |
4800 |
96.0-100.4 |
8681 |
10/107/207 |
13.3/4.3/3.7 |
4.9/2.4/3.6 |
aTwo MRM transitions, a quantifier (quan) and a qualifier (qual) were monitored for each analyte. The quantifier to qualifier ratio was used for peak identification based on
criteria set forth by the CLSI C50-A guideline |
