50:50 ANC:KPF
6
( 0.1 M), pH 3.2
T
FR
K
1
K
2
K
3
R
1
R
2
α
1
α
2
P
1
P
2
P
3
30
1.0
7.5
9.5
13.0
1.7
2.1
1.2
1.4
1564
1191
970
35
0.85
7.5
9.6
12.4
1.6
2.1
1.2
1.3
2278
1920
1536
37
0.65
9.1
10.6
13.5
1.6
2.3
1.2
1.3
2531
2134
1778
38
0.5
11.7
13.5
17.0
1.6
2.4
1.2
1.3
2911
2437
2082
Table 5:
The effect of Temperature and flow rate on retention factor, theoretical plate and enantioselectivity of Atracurium besylate isomers at pH 3.2.
