| Chromatographic parameter |
RSD % |
Tailing factor |
plate count |
sample |
| Result
(ppm) |
|
1) Wavelength (nm) |
|
|
|
|
| 250 |
0.27 |
1.2 |
5869 |
1.70 |
| 252 |
0.28 |
1.2 |
5874 |
1.30 |
| 254 |
0.42 |
1.2 |
5866 |
1.30 |
| 256 |
0.45 |
1.2 |
5870 |
1.30 |
| 258 |
0.42 |
1.2 |
5873 |
1.30 |
|
2) Flow rate (ml min-1) |
|
|
|
|
| 0.8 |
0.09 |
1.2 |
28089 |
1.45 |
| 0.9 |
0.05 |
1.2 |
6695 |
1.50 |
| 1 |
0.42 |
1.2 |
5866 |
1.30 |
| 1.1 |
0.07 |
1.1 |
6016 |
1.40 |
|
3) Column temperature (°C) |
|
|
|
|
| 20 |
0.27 |
1.1 |
6254 |
2 |
| 23 |
0.18 |
1.1 |
6435 |
1.4 |
| 25 |
0.42 |
1.2 |
5866 |
1.32 |
| 27 |
1.98 |
1.1 |
6632 |
1.62 |
| 4) % of Acetonitrile content in the mobile phase |
|
|
|
|
| 12.0% |
0.69 |
1.0 |
6092 |
2 |
| 15.0% |
0.42 |
1.2 |
5866 |
1.3 |
| 18.0% |
1.16 |
1.4 |
10988 |
2.3 |
| 20.0% |
1.75 |
1.5 |
14231 |
1.5 |
|
