| Chromatographic parameter |
RSD % |
Tailing factor |
plate count |
sampleresult (ppm) |
| 1)Flow rate (ml min-1) |
| 1.2 |
0.19 |
1.1 |
9925 |
0.148 |
| 1.3 |
0.18 |
1.1 |
9724 |
0.147 |
| 1.5 |
0.16 |
1.1 |
8962 |
0.149 |
| 1.6 |
0.08 |
1.1 |
8474 |
0.148 |
| 1.8 |
0.16 |
1.1 |
7564 |
0.148 |
| 2) Column temperature(°C) |
| 35 |
0.18 |
1.13 |
8573 |
0.146 |
| 37 |
0.50 |
1.12 |
8877 |
0.148 |
| 40 |
0.13 |
1.14 |
8361 |
0.149 |
| 43 |
0.32 |
1.13 |
8365 |
0.148 |
| 45 |
0.14 |
1.13 |
8272 |
0.149 |
| 3) % of Acetonitrile content in the mobile phase |
| 31.0% |
0.15 |
1.18 |
7262 |
0.146 |
| 30.0% |
0.09 |
1.15 |
7709 |
0.149 |
| 29.0% |
0.13 |
1.14 |
8361 |
0.143 |
| 27.0% |
0.11 |
1.12 |
9549 |
0.139 |
| 26.0% |
0.18 |
1.10 |
10179 |
0.135 |
|
