Figure 7: Comparison between Hypercum hyrcinum (tracks 1-4) and H. perforatum (5-9) with selected standards including hypericine (9). Mobile phase: formic acid, water, ethyl acetate (10:5:85). Derivatization: the layers were treated with a solution containing the Natural Product Reagent (NPR) (1 g diphenylborinic acid aminoethylester in 200 mL of ethylacetate), dried in the open air and then dipped into Macrogol reagent (1 g polyethylene glycol 400 in 20 mL of dichloromethane). Visualization: UV254 in the upper plate and at UV366 the lower plate.