Time (min) % A, 0.1% v/v TEA Buffer %B, Acetonitrile 0.00 70 30 0.30 70 30 0.50 64 36 0.90 64 36 1.30 40 60 2.00 40 60 2.05 70 30 2.20 70 30

Table 2: Gradient program.