| S.No. |
Robustness parameter |
% RSD for AMB |
%RSD for ASC |
| 1 |
Flow rate–10% (0.72 ml/min) |
0.560 |
0.752 |
| 2 |
Flow rate +10% (0.88 ml/min) |
0.720 |
0.430 |
| 3 |
pH+0.05 (3.05) |
0.672 |
1.50 |
| 4 |
pH–0.05 (2.95) |
1.502 |
1.212 |
| 5 |
Column oven temperature-5°C (40°C) |
0.620 |
0.510 |
| 6 |
Column oven temperature+5°C (50°C) |
0.320 |
0.481 |
| 7 |
Organic phase–2 from absolute value |
0.369 |
1.034 |
| 8 |
Organic phase +2 from absolute value |
0.651 |
0.832 |
