Figure 3: Denaturing SDS-PAGE and immunoblot analysis of chimeric conjugates: lane 1 MW marker, lane 2 SpLA-HRP (Sigma), lane 3 SpLAHRP (homemade), lane 4 SpLG-HRP (homemade), lane 5 SpG-HRP (home made), lane 6 SpAG-HRP (homemade), lane 7 SpLA-LG-HRP and lane 8 SpLAG-HRP (homemade). Immunoblot analysis showed that all chimeric conjugates reacted with human serum immunoglobulins and it also confirmed their polymeric nature.