Parameter Data
Linearity range( ng per spot) 500–3000
Limit of Detection ( ng ) 30
Limit of Quantitation ( ng ) 82
Accuracy (n = 9) 99.54±0.42
Precision (% RSD) Repeatability of application (n = 7) 1.65
Interday (n = 3) 0.49
Intraday (n = 3) 0.78
Specificity Specific
Robustness Robust
*mean of three estimations
Table 3: Summary of validation parameters of developed HPTLC method.