Parameter S.D. of peak area %R.S.D.
Mobile phase composition 92.32 0.33
Amount of mobile phase 60.44 0.92
Temperature 71.08 0.62
Relative humidity 81.02 1.28
Plate pretreatment 84.25 1.27
Time from spotting to chromatography 96.51 1.61
Time from chromatography to scanning 88.31 1.29
Table 4: Results from the robustness study of method.