Spiked concentration (ng/400 μL)
Observed concentration (mean* ± S.D.
a
)
Coefficient of variation (%)
Accuracy (%)
Intra-day Dofetilide (n=10)
10
10.63 ± 0.50
4.73
106.29
25
23.55 ± 0.72
3.06
94.18
100
99.89 ± 3.76
3.76
99.86
Intra-day Amlodipine (n=10)
25
21.35 ± 1.67
7.82
85.40
100
90.59 ± 2.96
3.26
90.59
500
513.09 ± 21.61
4.21
102.62
a
Standard deviation
*Linear regression equations used: y=0.0060x for dofetilide and y=0.0017x for amlodipine
Table 2:
Intra-dayprecision and accuracy for determination of dofetilide and amlodipine in human plasma by the HPLC method.
