Parameters Spectrophotometric method RP-HPLC method PE LC TM PE LC TM L.P Mixturesa 98.71 ± 0.940 98.55 ± 1.030 99.19 ± 0.938 98.68 ± 1.196 99.33 ± 1.497 100.39 ± 1.285 Chromoderm® solutionb (B.No.A0380209) 97.82 ± 1.104   101.41 ± 0.660   99.65 ± 0.578   97.87 ± 0.573 101.05 ± 1.102 100.28 ± 0.983 Standard additiona 99.72 ± 0.569 99.96 ± 0.724 98.97 ± 0.519 97.96 ± 1.107 98.82 ± 0.997 98.93 ± 0.543 Degree of freedomc F-test 10 (3.178) 2.363 10 (4.657) 3.178 7 (4.283) 1.588 8 (4.387) 4.202 7 (3.153) 2.418 7 (3.971) 3.605 Degree of freedomc Student’st- test 10 (2.100) 0.826 10 (2.100) 0.143 7 (2.179) 0.848 8 (2.200) 0.227 7 (2.231) 0.599 7 (2.178) 0.627 aAverage of 3 determinations. bAverage of 6 determinations cThe values in the parenthesis are the corresponding theoretical values at p=0.05. cReported RP-HPLC method for PE and LC using Nucleosil5 C18 and methanol-0.01 M sodium dihydrogen phosphate (7:3) containing 0.005 M sodium dodecane sulphate as the mobile phase. cReported RP-HPLC for TM using anion exchange resin column and aqueous perchlorate solution as a mobile phase.

Table 1: Determination of the studied drugs in the laboratory prepared mixtures (L.P.) and pharmaceutical preparation by the proposed methods and statistical comparison with the reported RP-HPLC methods.