Parameters Obtained value Reference value  
PE LC TM
Resolution(Rs) 2.5   1.7 >1.5
Relative retention(α) 1.9   1.32 >1
Tailing factor(T) 1 1 1 T=1, for a typical symmetrical peak
Capacity factor(Ќ) 3.8 7.3 9.7 1-10 acceptable
Number of theoretical plates(n) 1825 2360 1685 Increase with the efficiency of the separation
HETPb 0.018 0.009 0.025 The smaller the value the higher the column efficiency
b=height equivalent to theoretical plate, (cm.plate-1).
Table 3: Statistical analysis of parameters required for system suitability testing of RP-HPLC.