| Validation parameters |
HPTLC-densitometry |
HPLC |
| Linearity range |
1.0-11.0 μg spot-1 |
1.0-30.0 μg ml-1 |
| Correlation coefficient (r) |
0.9997 |
0.9998 |
| Slope |
684.53 |
68.61 |
| Intercept |
-278.13 |
-2.24 |
| LOD |
0.29 μg spot-1 |
0.20 μg ml-1 |
| LOQ |
0.90 μg spot-1 |
0.56 μg ml-1 |
| Accuracy (Recovery% ± RSD) |
100.36 ± 1.41 |
100.21 ± 0.41 |
|
Precision (RSD%, n=9) |
Intra-day |
1.65 |
0.36 |
| Inter-day |
1.93 |
1.93 |
|
