Parameters |
HPTLC-densitometry |
HPLC |
Reference values |
QO |
DQ |
QTF |
QL |
QO |
DQ |
QTF |
QL |
|
tR,min (HPLC)/Rf(HPTLC) |
0.01 |
0.08 |
0.70 |
0.81 |
2.53 |
3.20 |
3.61 |
4.24 |
|
Tailing factor (T) |
0.90 |
0.76 |
0.93 |
1.02 |
0.73 |
0.96 |
1.01 |
1.18 |
T≤ 2 |
Plates number (N) |
|
3040 |
4608 |
4688 |
3991 |
N >2000 |
Height equivalent to theoretical plate (HETP; cm plate-1) |
|
0.049 |
0.032 |
0.031 |
0.037 |
The smaller the value, the higher the column efficiency |
Resolution (RS) |
16 |
10.47 |
- |
1.6 |
2.37 |
2.03 |
- |
2.64 |
RS≥ 2 |
|