| Condition |
Station |
USP Resolution (Rs) |
USP Tailing factor (T) |
Relative retention time (RRT) |
| Mobile phase composition change (%) |
90 |
9.11 |
1.16 |
1.41 |
| 100 |
8.58 |
1.13 |
1.39 |
| 110 |
8.23 |
1.14 |
1.38 |
| Column oven temperature(°C) |
22 |
8.48 |
1.12 |
1.40 |
| 27 |
8.58 |
1.13 |
1.39 |
| 32 |
7.95 |
1.10 |
1.36 |
| Column flow (mL/min) |
0.8 |
9.27 |
1.14 |
1.39 |
| 1.0 |
8.58 |
1.13 |
1.39 |
| 1.2 |
7.92 |
1.11 |
1.38 |
|
