UHPLC GC-MS LC-MS MALDI-TOF Immunoaffinity Size Exclusion Atmospheric Pressure/G 50,000 psi He, N2, H2 Gas-N2, psi depends on instrument Varies with instrument model Varies with instrument model Varies with instrument model Laser/UV Application UV Laser Laser, UV N2 Laser beam- 337, 355, 266 UV (280 nm) UV-280 nm, 230 nm, 210 nm, 410 etc., depends on samples Time (total experiment) 45 minutes 16 minutes 1.18/30.00 min Avg.1 hour, depends on plate size Avg. 30 minutes Avg. 30 minutes Matrix Core shell, hybrid particles, sephadex, 2, 3, 5, 10 micron particles Inert material or sand Phospholipid Varies depends on        biological samples Sepharose (90 µm), metals, hybrid metals and Various IgG’s Superdex/Sephadex Column (diameter and length) diameter-1-2 mmLength-50-250 mm Capillary glass, steel, copper column- 30-60 meter 0.1-1 mm or 150 mm NA 1-5 ml 150 × 2.1 mm 4.6 × 50-150 mm 3.2 × 100 mm Elution Solvent Acetonitrile,  Formic acid,  Methanol Acetonitrile, Formic Acid, Water Ammonium Acetate, Formate, Hydrogen Carbonate, Formic/Acetic Acid, Trifluoroacetic acid, Trialkylamine, TRIS, BIS-TRIS, propane Acetonitrile, Water,     Methanol, Chloroform, Propanol, Ethanol Tris-HCL, NaCl Acetonitrile in TFA

Table 1: A comparative table of experimental procedures and parameters.