Species IgG Mean OD at 450 nm Standard Deviation (SD) Mule IgG 0.61 0.04 Human IgG (+ control) 0.81 0.02 Chicken IgY (- control) 0.14 0.008 Blank 0.06 0.003

Table 1: Results of the Protein A- Enzyme Linked Immunosorbent Assay (ELISA)for detection of mule IgG. This assay confirmed the previous purification procedure,since the SpA used only binds to IgG molecules.