Run time (min)
Flow rate (µL/min)
A (%)
B (%)
0.00
250
95.0
5.0
0.10
250
95.0
5.0
0.60
250
5.0
95.0
1.30
250
5.0
95.0
1.40
250
90.0
10.0
2.00
250
90.0
10.0
Table 1:
Chromatography gradient elution.
