Altered condition Retention time (min) Tailing Factor Theoretical plates % RSD %Assay
Normal (unaltered) (Repeatability) 11.6 1.19 44742 0.08 98.9
Flow rate of mobile phase (0.8 mL/min) 12.94 1.20 43095 0.08 100.5
Flow rate of mobile phase (1.2 mL/min) 10.61 1.18 47443 0.09 99.4
  pH of buffer of mobile  
phase (pH=2.3)
10.30 0.98 71000 0.07 100.0
  pH of buffer of mobile
phase (pH=2.7)
10.30 1.01 69703 0.03 100.8
Table 10: Robustness results of the proposed method (For Entacapone).