Altered condition Retention time (min) Tailing Factor Theoretical plates % RSD %Assay
Normal (unaltered) (Repeatability) 2.60 1.37 5402 0.15 101.15
Flow rate of mobile phase (0.8 mL/min) 3.22 1.36 5928 0.22 101.9
Flow rate of mobile phase (1.2 mL/min) 2.18 1.32 4493 0.12 102.3
  pH of buffer of mobile
phase (pH=2.3)
2.37 1.00 7508 0.11 100.4
  pH of buffer of mobile
phase (pH=2.7)
2.33 1.04 7097 0.08 99.4
Table 11: Robustness results of the proposed method (For Levodopa).