Altered condition Retention time (min) Tailing Factor Theoretical plates % RSD %Assay
Normal (unaltered) (Repeatability) 3.62 1.19 6330 0.10 95.9
Flow rate of mobile phase (0.8 mL/min) 4.51 1.18 6907 0.12 96.9
Flow rate of mobile phase (1.2 mL/min) 3.04 1.17 5204 0.12 96.6
  pH of buffer of mobile
phase (pH=2.3)
3.27 0.94 6436 0.94 96.0
  pH of buffer of mobile
phase (pH=2.7)
3.05 1.01 6201 0.09 96.5
Table 12: Robustness results of the proposed method (For Carbidopa).