Chromatographic parameters
Optimized conditions
Stationary phase
Enable HPLC C18 G 120A Column 250 ×4.6 mm, 5μ
Mobile phase
Methanol: water, 50:50 (%v/v)
Flow rate
1.2 ml/min
Injection volume
20μl
Detection wavelength
282 nm
Run time
10 minutes
Table 1:
Optimized Chromatographic Conditions for RP-HPLC.
