| Parameters |
Results |
| Calibration curve range |
1-16μg/ml |
| Regression line equation |
Y=1639x+50431 |
| Slope |
1639 |
| Intercept |
50431 |
| Correlation coefficient (r2) |
0.999 |
| Summary of Validation |
| Accuracy |
% Recovery |
% RSD |
| 80% |
98.87 |
0.495 |
| 100% |
98.95 |
1.340 |
| 120% |
99.46 |
0.330 |
| Precision |
Concentration(μg/ml) |
% RSD |
| Repeatability (n=6) |
8 |
1.205 |
| Intraday precision (n=3) |
4 |
0.986 |
| 8 |
0.920 |
| 12 |
1.08 |
| Interday precision (n=3) |
4 |
0.982 |
| 8 |
0.582 |
| 12 |
0.780 |
| LOD |
0.25μg/ml |
| LOQ |
0.75μg/ml |
| Robustness |
No significant changes observed with deliberate changes in the method parameters |
|
