Figure 4: Chromatogram showing separation of osmoregulants in Atriplex cinerea extract using (a) a NH2 column and a mobile phase consisting of 0.1% formic acid and ACN in gradient mode and (b) a C18 column and a mobile phase consisting of 0.1% HFBA and ACN in gradient mode. The extract was diluted 3 fold for the C18 separation. Peak identification: 2. betaine, 6. sucrose. Reprinted from Ref. [6], with permission.