Figure 6: Effect of different voltages on separation of NLB (100 μg mL-1); NLX(40 μg mL-1), NLT (40 μg mL-1) and 100 μg mL-1 IS; running buffer; 20 mmol L-1acetate buffer; injection 70 mbar for 6 s; separation voltage (15-45 kV); capillarytemperature 25°C and DAD detection at 230 nm.