Ratio
NLB:NLX:NLT
% w/w
Taken
μg mL-1
NLB Reference method [2]
Taken 2-12 μg mL-1
NLX Reference method [10]
Taken 10-100 μg mL-1
NLT Reference method [21]
Taken 12-32 μg mL-1
Found
μg mL-1
% Recovery Found
μg mL-1
% Recovery Found
μg mL-1
% Recovery Found
μg mL-1
% Recovery Found
μg mL-1
%
Recovery
Found
μg mL-1
%
Recovery
1:1:1
1:2:2
1:4:4
1:6:6
1:8:8
1:10:10
20:20:20
20:40:40
20:80:80
20:120:120
20:160:160
20:200:200
19.98
19.87
19.52
19.69
19.84
19.88
99.9
99.4
97.6
98.5
99.2
99.4
1.97
3.99
5.87
7.95
9.86
11.96
98.5
99.8
97.8
99.4
98.6
99.7
19.85
39.57
79.99
120.00
159.96
199.89
99.3
98.9
99.9
100.0
99.9
99.9
9.99
19.98
39.86
59.87
79.86
98.95
99.9
99.9
99.7
99.8
99.8
98.9
19.87
39.74
79.63
119.82
159.99
199.25
99.4
99.4
99.5
99.9
99.9
99.6
11.98
15.86
20.00
23.87
27.93
32.00
99.8
99.1
100.0
99.5
99.8
100.0
Mean ± SD
Variance
**%SE
t-test
F-test
99.00 ± 0.8
0.64
0.33
0.068(2.228)*
1.31(5.05)*
98.97 ± 0.7
0.49
0.29
99.65 ± 0.4
0.16
0.16
0.100(2.228)*
1.78(5.05)*
99.67 ± 0.3
0.09
0.12
99.62 ± 0.2
0.40
0.08
0.555(2.228)*
4.44(5.05)*  
99.70 ± 0.3
0.09
0.12
* Figures in parentheses are the tabulated values of t-and F- testes at 95% confidence limit [32]. ** %SE= SD/image.
Table 5: Analytical results of the determination of NLB, NLX and NLT in authentic mixture using CZE method and reference methods.