Calibration
Standard Identification

Volumes Used (mL)

Total Volume
(mL)

Final Concentration
(µg/mL)

100 or 1000
µg/mL Fe

Diluent

S0

0

1.000

1.000

0

S1

0.005 (100)

0.995

1.000

0.5

S2

0.010 (100)

0.990

1.000

1

S3

0.020 (100)

0.980

1.000

2

S4

0.005 (1000)

0.995

1.000

5

S5

0.010 (1000)

0.990

1.000

10

S6

0.015 (1000)

0.985

1.000

15

S7

0.018 (1000)

0.982

1.000

18

S8

0.020 (1000)

0.980

1.000

20

Table 1: Calibration Standard Preparation.