QC Sample Identification

Volumes Used (mL)

Total Volume (mL)

Fortified Iron Concentration
In Matrix (µg/mL)

100, 1000 or 10,000 µg/mL Fe

Matrix

QC Control (i.e., no Fe)

0.000

1.000

1.000

0

LLOQ

0.005 (100)

0.995

1.000

0.5

QC Low

0.015 (100)

0.985

1.000

1.5

QC Mid

0.010 (1000)

0.990

1.000

10

QC High

0.015 (1000)

0.985

1.000

15

QC Dilution

0.010 (10,000)

0.990

1.000

100

Table 2: QC Sample Preparation.