Figure 5: 20 µm frozen section of optic nerve of Nothobranchius guentheri probed with rabbit E587 antiserum and mouse anti-human GFAP, GA-5. (C) shows a merged image of the red and green channels. The blue line indicates the optical section used to generate the profile in (d) which shows co-localization of red and green signal in the glial processes. Scale bar = 20 µm.