Figure 2: Mannitol solution reduced necrosis and CD95 positivity after SCI. In A, regarding motor recovery, no improvement was observed within the initial 3 days after acute spinal cord injury; In B, semi-quantitative analysis of hematoxylin-eosin stained samples, according to the criteria in the methods, revealed significant reduction in degree of necrosis in gray matter in the experimental group that received mannitol. In white matter, no significant change was observed, perhaps due to the low number of cells. In C, semiquantitative analysis of CD95-positivity revealed significantly fewer positive cells in white and gray matter in the mannitol group.