Figure 3: The volume densities (Vv) of the collagen bundles, vessels and hair follicles were estimated by using a grid of points (designed at Histomorphometry & Stereology Research Centre, Shiraz University of Medical Sciences) on the live image of dermis. The total number of points hitting the collagen bundles or vessels or hair follicles is counted and divided by the total number of points hitting the reference space (dermis). The right upper corner of the cross, presented at the right corner of this figure, is considered as the point (arrow), and it is counted only if the right upper corner hits the tissue. (Hedenhain’s azan stain) (× 450).