Figure 2: Representative results of ultra-rapid quantitative RT-PCR of the serial extensive lavages by LightCycler. Curves and Lanes 1st-10th were 100-ml samples from the first to the tenth wash, each using 1 liter of saline. WiDr was a positive control containing 105 cells in 100 ml of saline, and DW contained no template. The free cancer cells in the lavage fluids were serially diluted by 8 liters of saline and disappeared in washing fluids after the eighth wash.