Figure 7: Representative photomicrographs of αSMA (x200) in renal tissue of control rat (A) or AA-treated rats at days 3 (B), 10 (D), 21 (E), and day 35 (F). Semiquantitative analysis of α-SMA-positive staining in control, SHAM- or AA-treated rats at different time-points after treatment (C). Double immunostaining for αSMA (green) and CD44 (red) (arrowhead) in AA-treated rats at day 10 (G,H x400 + insert to better illustrate the co-localization) and at day 35 (I, x400 – J, x1000). *p ≤ 0.05 versus SHAM; #p ≤ 0.05 versus control