Figure 4: The width of zona pellucida and follicular layer in relationship to follicle diameter. *1-statistically significant difference (p<0.05) in relation to D2, E2 and F2; *2-statistically significant difference in relation to C2, D2, E2 and F2; *3-statistically significant difference in relation to D2 and F2; *4-statistically significant difference in relation to A1, B1, C1, E1 and F1. *5-statistically significant difference in relation to A1, B1, C1, D1 and F1. Control group refers to follicles isolated from sexually mature females.