Follicle diameters (µm) Mean difference in width of layers (±SE) 301-600 601-1200 1201-2000 2001-5000 5001-10000 Follicular layer ≤300 3.28 ± 3.69 8.72 ± 3.73 19.19 ± 3.95** 13.95 ± 3.92* 18.06 ± 4.16** 301-600   5.45 ± 1.23** 15.91 ± 1.79** 10.67 ± 1.72** 14.78 ± 2.22** 601-1200     10.47 ± 1.88** 5.22 ± 1.82 9.33 ± 2.29** 1201-2000       5.24 ± 2.23 1.14 ± 2.63 2001-5000         4.11 ± 2.59 Zona pellucida ≤300 3.62 ± 3.87 7.33 ± 3.91 26.50 ± 4.14** 15.87 ± 4.11* 5.50 ± 4.63 301-600   3.70 ± 1.29 22.87 ± 1.87** 12.25 ± 1.80** 1.87 ± 2.79 601-1200     19.17 ± 1.97** 8.54 ± 1.90** 1.83 ± 2.86 1201-2000       10.63 ± 2.34** 21.00 ± 3.16** 2001-5000         10.38 ± 3.12*

Table 1: Changes in the width of follicular layer and the zona pellucida among compared ovarian follicles analysed in Hochberg’s GT2 post hoc test. *- significance at the level 0.05; **- significance at the level 0.001.