Figure 4: Phase-contrast photographs of SSEA-1+ cells with/without 5-azacytidine treatment after three weeks. (A) 5-azacytidine treated SSEA-1+ cells show an increase in size with no further proliferation. (B). Cells grow in differentiation medium only show cobblestone-like morphology with a slight increase in cell size and proliferation. (C) In the growth medium cells show spindle shape morphology and become confluent.