A: XAV939 decreased cellular levels of beta-catenin by increasing axin in MDAMB231 cells. The treatment with XAV939 (10 µM) (A) caused an increase in axin protein expression as well as a decrease in beta-catenin protein. β-actin was used as loading control.
B: Wnt signaling modulators (WntC59: 10 nM) (i) and (XAV939: 5 µM) (ii) blocked VM at 6 hours in EGFP-MDA-MB231BR cells. XAV939 (10 µM) mediated decrease in beta-catenin protein blocked VM in EGFP-MDA-MB231BR cells (Bi). The blockade of VM by XAV939 was observed at both 5μM and 10μM doses.WntC59 blocked VM in EGFP-MDA-MB231BR cells (B ii). The same zero hour control for EGFP-MDA-MB231BR was used for this data (B i & ii) and EGFPMDA-MB231BR VM standardization data in figure 1C. Insets show respective photomicrographs at 4X magnification
C: LY294002 (25µM) and SF1126 (25µM) decreased cellular levels of pGSK3beta in HCC1937, MDA231 and MDA-MB468 TNBC cells. Total GSK3beta was used as the specific loading control.
D: LY294002 and SF1126 blocked VM in MDA-MB231 and BT20 TNBT cells. Panels showed images of VM in different cell lines.
