Cell transfer technique |
(1) Dilute Malinol with an equal volume of xylene. |
(2) Cover the glass slide after detaching the cover slip with 1 ml of diluted Malinol. |
(3) Place Malinol solidified on an 80°C hot plate for 30 min. |
(4) Dip the solidified thin membrane into 60°C water for 15 min to soften it. |
(5) Peel off the softened Malinol membrane with forceps. |
(6) Trim the membrane into pieces. |
(7) Place the trimmed pieces on new silane-coated glass slides in warm water. |
(8) Remove excess air bubbles and water using tissue paper. |
(9) Dry glass slides on an 80°C hot plate for 30 min. |