Figure 1: A copy of a display seen on the monitor’s screen of the image analyzer of a cross section of rat jejunal specimen of methotrexate treated group showing the: a) optic density of alkaline phosphatase enzyme activity masked by blue color inside the frame. (DAB x 400). b) areas of connective tissue fibres masked by blue color inside the standard frame. (Masson’s Trichrom x 400). c) method of measuring the villus height (blue double head arrows) and crypts depth. (yellow double head arrows). (H & E x 100). d) method of counting the goblet cells. (H & E x 400).