Figure 1: Left panels: Representative example of flow cytometry quantification of the CD11R3 surface marker expression on swine blood monocytes. A: Dual-parameter dot-plot identification of the monocyte cluster (R1) based on side light scatter (SSC) properties and the bright fluorescence for the pan-myeloid marker CD172a (FL3). The R1-derived overlaid histograms subtraction (test histogram–control histogram) (B) has been used to quantify the percentages of positivity for CD11R3 (events in M1, filled histogram) and its relative fluorescence intensity (RFI) (linear scale) with respect to the corresponding isotype controls (dotted histogram). Right panels: Flow cytometry quantification of the circulating CD14low/CD163high monocyte fraction (as percentage). A: The monocytes have been clustered as previously described. Based on the median channel (linear scale) of the R1-derived CD14 fluorescence distribution (FL1, B), a dual-parameter dot-plot quadrant of FL2 vs FL1 has been used to quantify the percentage of CD14low/CD163high monocytes (upper left quadrant, D) compared with the corresponding isotype control (upper left quadrant, C).