Test Observation Inference Alkaloid (i) 1ml of extract + few drops of Drangendoff’s reagent Yellowish brown colour Alkaloid inferred (ii) 1ml of extract + few drops of Mayers reagent Yellowish colour seen Alkaloid inferred (iii)1ml of extract + Wagner’s reagent Dark turbid brown Alkaloid inferred

Table 1: Biochemical tests for Alkaloids presence