Figure 5: Representative micrographs of two cases of low grade astrocytomas immunostained for nestin, SPARC and IDH1(R132H). Case 1 expressed the mutated IDH1 protein, whereas case 2 was clearly IDH1(R132H)-negative. Both cases were nestin and SPARC immunopositive. The center (c) and periphery (p) of the corresponding tumors are depicted for each case. Original magnification x400. Scale bar: 100 μm.