Figure 6: Representative micrographs of two cases of anaplastic astrocytoma immunostained for nestin, SPARC and IDH1(R132H). Case 1 was positive for all three markers, whereas case 2 was IDH1(R132H) negative and low for SPARC expression. For both cases, the center (c) and periphery (p) of the tumors are depicted. Original magnification x400. Scale bar: 100 μm.