Figure 8: Representative micrographs of the tumor centers of two cases of sGBM with low SPARC immunostaining. The two cases (case 1 and 2) showed high cellularity, with strong nuclear expression of PCNA and p53 in almost all cells, in addition to the expression of IDH1(R132H). Case 1 showed low number of nestin-positive cells, whereas case 2 revealed strong nestin immunostaining in all cells at the “hot spots” indicated by black arrowheads. Original magnification x400. Scale bar: 100 μm.