Antibody against Clone Source1 Concentration Pretreatment Technique2 β-Catenin 14 BD Biosciences 0.6 mg/ml Press. cooker, citrate HRP-DAB bcl-2 124 Dako 7.5 mg/ml Press. cooker, citrate HRP-DAB bcl-6 PG-B6p Dako 19.5 mg/ml Press. cooker, citrate HRP-DAB c-erbB2 polyclonal Dako 1:500 Press. cooker, citrate HRP-DAB CNPase 11-5B abcam 1 :1600 Microwave, citrate HRP-DAB Cyclin D1 P2D11F11 Novocastra 6.0 mg/ml Microwave, urea HRP-DAB E-Cadherin HECD-1 R&D Systems 1.0 mg/ml Press. cooker, citrate AP-NF EGFR 5B7 Ventana Ready to use Pronase HRP-DAB GFAP 6F2 Dako 2.6 mg/ml Microwave, citrate HRP-DAB Hey1 polyclonal abcam 1:500 No pretreatment HRP-AEC IDH1(R132H) H09 dianova 1:20 Microwave, citrate HRP-DAB MAP2 HM-2 Sigma-Aldrich 1:20000 Tris-EDTA pH 8.5 HRP-DAB MIB1 MIB1 Dako 1.6 mg/ml Press. cooker, citrate HRP-DAB Nestin 10C2 Serotec 1:50 Tris-EDTA pH 8.5 HRP-DAB Neurofilament 2F11 Dako 5.4 mg/ml Press. cooker, citrate AP-NF Osteonectin 15G12 Leica 1:500 Microwave, citrate HRP-DAB p53 DO-7 Dako 1.0 mg/ml Press. cooker, citrate HRP-DAB PCNA PC10 Dako 7.7 mg/ml Microwave, citrate HRP-DAB Sox2 6F1.2 Millipore 1:100 No pretreatment HRP-AEC STAT3-α polyclonal Cell Signaling 1:200 Microwave, urea HRP-DAB Substance P polyclonal BioGenex 1:1600 Microwave, citrate HRP-DAB Synaptophysin Snp88 BioGenex 1:100 Press. cooker, citrate AP-NF Tenascin C 49 Leica 1:50 Microwave, citrate+trypsin HRP-DAB Vimentin 3B4 Dako 0.5 mg/ml Trypsin HRP-DAB 1Manufacturer locations: abcam, Cambridge, UK; BD Biosciences, Basel, Switzerland; BioGenex, San Ramon, CA, USA; Cell Signaling, Beverly, MA, USA; Dako, Glostrup, Denmark; Dianova, Hamburg, Germany; Leica, Nünningen, Switzerland; Millipore, Merck Millipore, Darmstadt, Germany; Novocastra, Newcastle-upon-Tyne, UK; R&D Systems, Minneapolis, MN, USA; Serotec, Düsseldorf, Germany; Sigma-Aldrich, St-Louis, MO, USA; Ventana Medical Systems, Tucson, AZ, USA. 2Techniques: AP-NF: ABC method using alkaline phosphatase and new fuchsin; AEC: 3-amino-9- ethylcarbazole; HRP-DAB: ABC method using horseradish peroxidase and 3,3'-diaminobenzidine (DAB) or OptiView DAB detection kit/Benchmark XT (Ventana Medical Systems).

Table 1: Primary antibodies used for immunohistochemistry and technical information.