| Gene name |
Forward primer(5’→3’) |
Reverse primer(5’→3’) |
Product size/bp |
Tm/°C |
| PITX2 |
CTCCAGTCTCAACAGCCTGA |
ATACTGGCAAGCACTCAGGT |
226 |
59.0 |
| LEF-1 |
AAACCGAAGCGAAAGGGAAG |
AACGAAACGTCCACTTCCTG |
113 |
58.0 |
| β-catenin |
TGGGACACAGCAGCAATTTG |
AGCAGCTGCACAAACAATGG |
147 |
60.0 |
| GAPDH |
AGAAGGCTGGGGCTCATTTG |
AGGGGCCATCCACAGTCTTC |
258 |
60.0 |
|
